FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22965
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC606026.3879817112998No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC538523.448726322664925No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT335414.604833442194645No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC326614.221641628565207No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG9984.345743522752014No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT7593.305029392553886No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG1080.47028086218158066No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG1010.43979969518833006No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT920.400609623339865No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG730.3178750272153277No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA640.2786849553668626No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT460.2003048116699325No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT450.1959503592423253No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC450.1959503592423253No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT380.1654691922490747No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA370.16111473982146743No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT310.13498802525582407No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT260.11321576311778794No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA250.10886131069018071No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA250.10886131069018071No Hit
GGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT250.10886131069018071No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT240.10450685826257348No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC230.10015240583496625No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT230.10015240583496625No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)