FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9215
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC379141.13944655453066No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT173518.827997829625613No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG110011.937059142702116No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT96710.493760173629951No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG1431.551817688551275No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG760.8247422680412372No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC460.4991861096039067No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA430.4666304937601736No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC400.4340748779164406No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC350.3798155181768855No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT250.27129679869777534No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA240.26044492674986436No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA220.23874118285404233No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT190.2061855670103093No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT190.2061855670103093No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTCCGTTGAGGTGTAGAACCATGTCGT180.19533369506239828No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA150.16277807921866522No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCGTTGAGGTGTAGAACCATGTCGT140.1519262072707542No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT140.1519262072707542No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCCCCAGCTTATCATTCAC130.1410743353228432No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA130.1410743353228432No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA130.1410743353228432No Hit
TGGCTCATCTTCAAACCGTCCCCTGTTTTGTAGTCCAACCCTGTGATGAT120.13022246337493218No Hit
GCTACCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA120.13022246337493218No Hit
GCTATCCTGCCATGCTCCAGACACTCCTACAGCTCCAGCTTATCATTCAC120.13022246337493218No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAACTCCAGCTTATCATTCAC120.13022246337493218No Hit
TGGCTCATCTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT110.11937059142702117No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCCTATCATTCAC110.11937059142702117No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA110.11937059142702117No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATCCAC100.10851871947911015No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCGGCTCCAGCTTATCATTCAC100.10851871947911015No Hit
CTTCCTCTTCTCAGCAGTTGTGTTTCCGTTGAGGTGTAGAACCATGTCGT100.10851871947911015No Hit
GCTATCCTGCCTGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA100.10851871947911015No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)