FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23329
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC678629.088259248146088No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC518822.238415705773928No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT376416.134424964636292No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC282112.092245702773372No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG12605.4010030434223495No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT4541.946075699772815No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT990.42436452484032755No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG910.3900724420249475No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG730.3129152556903425No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA680.29148270393073No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC670.2871961935788075No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA440.18860645548458999No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT430.1843199451326675No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT430.1843199451326675No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT380.162887393373055No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA350.15002786231728749No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT340.145741351965365No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA290.1243088002057525No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA280.12002228985382998No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC280.12002228985382998No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT270.1157357795019075No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)