FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15439
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC913959.19424833214586No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT368723.881080380853682No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC5303.4328648228512213No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT920.5958935164194572No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT890.5764622061014315No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT730.4728285510719606No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC680.44044303387525097No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC550.35624068916380597No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT500.3238551719670963No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT480.3109009650884125No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT460.2979467582097286No Hit
ACAACAGGTCAGATGCAGGTCCAGCGGTGTCCCAAGCTCCCACCCAGCGC460.2979467582097286No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT450.29146965477038667No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT430.2785154478917028No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT370.23965282725565126No Hit
ACAACAGGTCAGATGCAGGCCCAGTGGTGTCCCAAGCTCCCACCCAGCGC310.20079020661959973No Hit
ACAACAGGTCAATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT280.18135889630157392No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT270.17488179286223202No Hit
ACAACAGGTCAGATCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT260.16840468942289008No Hit
ACAACAGGTCAGTGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT250.16192758598354814No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC250.16192758598354814No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCCCCCACCCAGCGC240.15545048254420624No Hit
ACAACAGGTCAGAGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT230.1489733791048643No Hit
ACAACAGGTCAGATGCAGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT220.1424962756655224No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGTGC200.12954206878683852No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGCCCCAAGCTCCCACCCAGCGC200.12954206878683852No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAACTCCCACCCAGCGC190.12306496534749661No Hit
AGACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT180.11658786190815466No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGCGTCCCAAGCTCCCACCCAGCGC180.11658786190815466No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGTTCCCACCCAGCGC170.11011075846881274No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCAC170.11011075846881274No Hit
ACAACAGGTCAGATGCAGGCCCGGCGGTGTCCCAAGCTCCCACCCAGCGC170.11011075846881274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)