FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30583
Total Bases4.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC873128.548540038583525No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT503616.466664486806394No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC430214.066638328483144No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT420713.756008239871825No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC25718.40663113494425No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG13374.371709773403524No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG1340.43815191446228297No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG1270.41526338161723836No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA890.29101134617271035No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG720.23542490926331622No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT690.22561553804401135No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC660.21580616682470655No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT560.18310826276035705No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT520.17002910113461728No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA480.15694993950887748No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA420.1373311970702678No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT420.1373311970702678No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC410.13406140666383284No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT360.11771245463165811No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC350.11444266422522317No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA340.11117287381878821No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC320.10463329300591832No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT320.10463329300591832No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)