FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17318
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC749443.27289525349347No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC290016.745582630788775No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT225713.03268275782423No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT14288.24575586095392No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC8905.139161566000693No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT830.4792701235708512No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC790.456172768218039No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT660.3811063633213997No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT580.3349116526157755No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT490.28294260307194824No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC460.2656195865573392No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT360.20787619817530892No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT340.19632752049890287No Hit
ACAACAGGTCAATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT330.19055318166069984No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT310.1790045039842938No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT300.17323016514609077No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT300.17323016514609077No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT260.15013280979327867No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT260.15013280979327867No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGCGTCCCAAGCTCCCACCCAGCGC250.14435847095507565No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT240.13858413211687262No Hit
ACAACAGGTCAGATGCAGGCCCAGTGGTGTCCCAAGCTCCCACCCAGCGC240.13858413211687262No Hit
ACAACAGGTCAGTGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT220.12703545444046657No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGATCTCT210.12126111560226355No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT200.11548677676406051No Hit
ACAACAGGTCAGATGCAGGTCCAGCGGTGTCCCAAGCTCCCACCCAGCGC200.11548677676406051No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT200.11548677676406051No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCCCCCACCCAGCGC190.1097124379258575No Hit
ACAACAGGTCAGATCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT180.10393809908765446No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.10393809908765446No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGCCCCAAGCTCCCACCCAGCGC180.10393809908765446No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)