FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33623
Total Bases4.8 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA1177035.00579960146328No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC715221.271153674568005No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT381711.352348095053982No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT356210.59393867293222No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC18945.633048805876929No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC14754.386878029919996No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC1110.3301311602177081No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT870.2587514499003658No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT800.23793236772447435No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT670.19926835796924722No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT650.19332004877613537No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA610.18142343038991166No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC550.1635785028105761No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT530.15763019361746425No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT480.1427594206346846No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT470.13978526603812866No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT410.1219403384587931No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA400.11896618386223717No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT400.11896618386223717No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT370.11004372007256938No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT370.11004372007256938No Hit

[WARN]Adapter Content

Adapter graph