FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780
Total Bases113 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC56272.05128205128204No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC567.179487179487179No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC374.743589743589744No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT141.7948717948717947No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT91.153846153846154No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT50.641025641025641No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC50.641025641025641No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT50.641025641025641No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT40.5128205128205128No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC30.38461538461538464No Hit
CGGAAACAACAATGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT30.38461538461538464No Hit
CGGAAACAACAACTGTTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC30.38461538461538464No Hit
CGGAAACAACAACCGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC20.2564102564102564No Hit
CGGAAACAACAACTGCTGAGATGAGGAAGAACTGGAGACCAAGTCTCTGC20.2564102564102564No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGA20.2564102564102564No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT20.2564102564102564No Hit
CGGAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT20.2564102564102564No Hit
CGGAAACAACAACTGCTGAGAAGAGGAGAACTGGAGACCAAGTCTCTGCT20.2564102564102564No Hit
CGGAAACAACAACTACTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC20.2564102564102564No Hit
CGGAAACAACAACTGGAGCATGGCAGGATAGCAACTGGAGACCAAGTCTC20.2564102564102564No Hit
CGGAAACAACAACTGCTGAGAAGAGGTAGAACTGGAGACCAAGTCTCTGC20.2564102564102564No Hit
CGGAAACAACAATTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
ACAACAGGTCAGATGCAGGCCAATCGCTCTCCCAAGATCCCACCCAGCGC10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAAGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
CGGAAATAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGGAAACAAAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
CTGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGA10.1282051282051282No Hit
CGAAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGGGGAAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGGAAACAACAACTGCTGACAAGAGGAAGAACAGGAGACCAACACTCAGC10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGAGGATGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGGAAACAACAACTGCTGCGAAGAGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
ACAACAGGTCAATGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA10.1282051282051282Clontech Universal Primer Mix Long (96% over 25bp)
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACAAAGTCTATGC10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGAGGGAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
ACAACAGGTCAGATGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.1282051282051282Clontech Universal Primer Mix Long (96% over 25bp)
ACAACAGGTCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.1282051282051282Clontech Universal Primer Mix Long (100% over 23bp)
CGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAAAGGAAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CAGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAACACTCAGC10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCACTGC10.1282051282051282No Hit
ACAACAGGTCAGATGCAGGGTACATGGGAAGCAGTGGTATCAACGCGGAG10.1282051282051282Clontech Universal Primer Mix Long (95% over 22bp)
CGGAAACAACAACTGCTGCATGGCAGATAGCAACTGGAGACCAAGTCTCT10.1282051282051282No Hit
CAACAGGTCAATGCAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.1282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
CGGAAACAACAACTGCCGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
GGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGTGGAAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGGAAACACAACTGCTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCT10.1282051282051282No Hit
CGGAAACAACAACTGCTGAAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
GGAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCTA10.1282051282051282No Hit
CGGAAACACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAAAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGAAACAACACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCTA10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGAGGAACTGGAGACCAAGTCTCTGCTAC10.1282051282051282No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
ACAACAGGTCAGATGCAGGGTACATGGGAAGCAGTGGTATCAACGCAGAG10.1282051282051282Clontech Universal Primer Mix Long (100% over 22bp)
CGGAAACAACAACTGCTGGAGCATGGCAGGATAGCAACAGGAGACCAAGT10.1282051282051282No Hit
CGGAACACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCTA10.1282051282051282No Hit
ACAACAGGTCAATGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.1282051282051282Clontech Universal Primer Mix Long (100% over 23bp)
CGGAAACAACAACTGCTGAGAAGAGAGAACTGGAGACCAAGTCTCTGCTA10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCAGC10.1282051282051282No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACAAAGTCTCTGC10.1282051282051282No Hit
CGGAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
ACAAAGGTCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.1282051282051282Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CGGAAAAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
CGGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTG10.1282051282051282No Hit
CGGAAACAACAACTGCTGCGAAGAGGATGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGGGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGAACTGGAGACCAAGTCTCTGCT10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGCAGAGGATGACCTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGTTAGTGACAAGAGGGACATCTGAGAAGAGGAAGAACTGGAGACCAAGT10.1282051282051282No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGACTCTGC10.1282051282051282No Hit
CGGAAACGACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.1282051282051282No Hit
CGGAAACACAATGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCTA10.1282051282051282No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGACTCTGC10.1282051282051282No Hit
CGGAAACACACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCTA10.1282051282051282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)