FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30394
Total Bases4.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC994732.726853984339016No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA716023.557281042310983No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT407813.417121800355334No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT370812.19977627163256No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC18306.02092518260183No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC1080.355333289465026No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT1010.33230242811081134No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT770.2533394748963611No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT770.2533394748963611No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT670.22043824439034018No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT600.19740738303612557No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT520.1710863986313088No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC520.1710863986313088No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT480.15792590642890045No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT460.15134566032769625No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT450.14805553727709417No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT450.14805553727709417No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT440.14476541422649208No Hit
ACAACAGGTCAGATGCAGGCCCAGTGGTGTCCCAAGCTCCCACCCAGCGC440.14476541422649208No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT390.1283147989734816No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT350.11515430677107323No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT350.11515430677107323No Hit
ACAACAGGTCAGTGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT340.11186418372047115No Hit
ACAACAGGTCAGATGCAGGTCCAGCGGTGTCCCAAGCTCCCACCCAGCGC330.10857406066986906No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCCCCCACCCAGCGC330.10857406066986906No Hit
ACAACAGGTCAATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT320.10528393761926695No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA310.10199381456866487No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)