FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26698
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC1203645.08202861637576No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA748628.039553524608586No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT18056.760806052887856No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC13475.045321746947337No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGATCTCT4591.7192299048617874No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT1440.5393662446625216No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC1150.43074387594576374No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT1000.37455989212675106No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT970.3633230953629485No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC710.2659375234099932No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT680.2547007266461907No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT640.23971833096112066No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT590.22099033635478313No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT550.20600794066971312No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT510.19102554498464305No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT500.18727994606337553No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT500.18727994606337553No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT490.18353434714210803No Hit
ACAACAGGTCAGTGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT440.16480635253577047No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA430.16106075361450298No Hit
ACAACAGGTCAGATGCAGGTCCAGCGGTGTCCCAAGCTCCCACCCAGCGC430.16106075361450298No Hit
ACAACAGGTCAGATGCAGGCCCAGTGGTGTCCCAAGCTCCCACCCAGCGC420.15731515469323545No Hit
ACAACAGGTCAATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT410.15356955577196793No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT400.14982395685070043No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA380.1423327590081654No Hit
ACAACAGGTCAGAGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT360.1348415611656304No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGCGTCCCAAGCTCCCACCCAGCGC360.1348415611656304No Hit
ACAACAGGTCAGATGCAGGCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT350.13109596224436287No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGCCCCAAGCTCCCACCCAGCGC320.11985916548056033No Hit
ACAACAGGTCAGATGCAGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT300.11236796763802533No Hit
ACAACAGGTCAGATCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT280.1048767697954903No Hit
TGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA280.1048767697954903No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)