FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29488
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA782026.519262072707544No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC584419.81823114487249No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC473716.064161692892025No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT340911.560634834508953No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT28679.722599023331524No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10923.7032013022246337No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC1220.41372761801410746No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT630.21364622897449811No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT580.19669017905588715No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT560.18990775908844276No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT550.18651654910472057No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC540.18312533912099838No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT540.18312533912099838No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT540.18312533912099838No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT540.18312533912099838No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA380.12886597938144329No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT340.11530113944655453No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT340.11530113944655453No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT330.11190992946283233No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA310.10512750949538796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)