FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14822
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC531635.865605181486984No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT386026.042369450816356No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC302620.41559843475914No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG5523.7241937660234785No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT790.5329914991229254No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT490.3305896640129537No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG430.2901092969909594No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT400.2698691134799622No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT390.26312238564296314No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG280.18890837943597355No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT280.18890837943597355No Hit
GGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT280.18890837943597355No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT240.16192146808797733No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC200.1349345567399811No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT200.1349345567399811No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT190.12818782890298205No Hit
TGGCTCATCTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT180.12144110106598299No Hit
AGACCCCTGGAACTGCTACATGTGCGGACACAAGGGTACCTACGGGCTGC180.12144110106598299No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCAGTGATGAT180.12144110106598299No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT180.12144110106598299No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCCGC170.11469437322898393No Hit
AGACCCCTGGAACTGTTACATGTGCGGGCACAAGGGTACCTACGGGCTGC170.11469437322898393No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACGAGGGTACCTACGGGCTGC160.1079476453919849No Hit
TGGCTCATCTTCAAACCGTCCCCTGTTTTGTAGTCCAACCCTGTGATGAT160.1079476453919849No Hit
TGGCTCATTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT160.1079476453919849No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTAC160.1079476453919849No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT160.1079476453919849No Hit
TGGTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT160.1079476453919849No Hit
TGGCTCATCTTCAAACGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT150.10120091755498584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)