FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35830
Total Bases5.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC1277235.646106614568794No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC533914.900921015908455No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT440612.296957856544795No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT380610.622383477532795No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC32339.023164945576333No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG10522.9360870778677084No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG2110.5888919899525538No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA1550.4325983812447669No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG1260.35166061959252026No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC1230.34328774769746023No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTTGAGGTGTAGAACCATGTCG960.2679319006419202No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA770.2149037119732068No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA750.20932179737650014No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT750.20932179737650014No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA620.17303935249790678No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT590.16466648060284678No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA520.14512977951437342No Hit
GCTATCCTGCCATGCTCCAGACACTCCCGCAGCTCCAGCTTATCATTCAC430.12001116382919341No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCGGCTCCAGCTTATCATTCAC420.11722020653084009No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT390.10884733463578007No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC390.10884733463578007No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA380.10605637733742675No Hit
GCTATCCTGCCATGCTCCAGGCACTCCTGCAGCTCCAGCTTATCATTCAC360.10047446274072007No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT360.10047446274072007No Hit
GCTACCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA360.10047446274072007No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT360.10047446274072007No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC360.10047446274072007No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA360.10047446274072007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)