FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816
Total Bases118.3 kbp
Sequences flagged as poor quality0
Sequence length144-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC65480.14705882352942No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC222.696078431372549No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGC202.450980392156863No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT131.5931372549019607No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT91.1029411764705883No Hit
CGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT60.7352941176470588No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGAACTGGAGACCAAGTCTCTGCT50.6127450980392157No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT40.49019607843137253No Hit
CGGAAACAACAACTGCTGAGAAGAGGAGAACTGGAGACCAAGTCTCTGCT40.49019607843137253No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAAACTGGAGACCAAGTCTCTGCT40.49019607843137253No Hit
CGGAAACAACAACTGCTGCATGGCAGGATAGCAACTGGAGACCAAGTCTC30.3676470588235294No Hit
CGGAAACAACAACTACTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC30.3676470588235294No Hit
CGGAAACAACACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT30.3676470588235294No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCAGC30.3676470588235294No Hit
CGGAAACAAAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT20.24509803921568626No Hit
CGGAAACAACAACTGCTGAGAGAGGAAGAACTGGAGACCAAGTCTCTGCT20.24509803921568626No Hit
CGGAACAACAACTGCTGCGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT20.24509803921568626No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAAAACTGGAGACCAAGTCTCTGC20.24509803921568626No Hit
CGGAAACAACAACTGCTGAGAAGGGAAGAACTGGAGACCAAGTCTCTGCT20.24509803921568626No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT20.24509803921568626No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGACTCTGC20.24509803921568626No Hit
CGGAAACAACAATTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTCCT10.12254901960784313No Hit
AGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAAGGAAGAACTGGAGACCAAGTCTCTGCT10.12254901960784313No Hit
CGGAAACAACAACTGCTGACACTGACGACATGGTTCTACACCTCAACGGA10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGAACTGGAGACCAAGTCTCTGCTACC10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTCC10.12254901960784313No Hit
CGGAAACAACAACTGCTGCGAAGAGGAGAACTGGAGACCAAGTCTCTGCT10.12254901960784313No Hit
CGGAAACAACAACAGCTGAGAAGACGAAGAACTGGAGACCAACTCTCAGC10.12254901960784313No Hit
CGGAAACAACAACTGGTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CGGAAAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCTA10.12254901960784313No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC10.12254901960784313No Hit
GGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCA10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGA10.12254901960784313No Hit
CGGAAACAACAACTGCTGGAGCATGGCAGGATAGCAACTGGAGACCAAGT10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGACGAAGAACTGGAGACCAAGACTCTGC10.12254901960784313No Hit
ACAACAGGTCAGATGCAGTTCCAGGGGTCTAACTGGAGACCAAGTCTCTG10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGGGAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGGGAAGAACTGGGGACCAAGTCTCATCT10.12254901960784313No Hit
CGGAACAACAACTGCTGAGAAGAGGAAAACTGGAGACCAAGTCTCTGCTA10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGAAGCACTGGAGACCAAGTCTCTGCT10.12254901960784313No Hit
CGGGAAAAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CGGAACAACAACTGCTGAGAAGAGAAGAACTGGAGACCAAGTCTCTGCTA10.12254901960784313No Hit
CGGAAACAACAACTGCCGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CGGAAACACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCAGCT10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGAAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CCGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CGGAAACAACAACTGCTGAAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.12254901960784313No Hit
CGGAAAAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.12254901960784313No Hit
CGAAACAACAACTGCTGAGAAGAGAAGAACTGGAGACCAAGTCTCTGCTA10.12254901960784313No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAAAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
ACAACAGGTCAGATGCAGGGTACATGGGAAGCAGTGGTATCAACGCAGAG10.12254901960784313Clontech Universal Primer Mix Long (100% over 22bp)
CGGAAACAACAACTGCTGACAAGAGGAAGAACTGGAGACCAAGTCTCAGC10.12254901960784313No Hit
CGGAACACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCTA10.12254901960784313No Hit
CGGAAACAACAACTAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTG10.12254901960784313No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTACTGCT10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACAAAGTCTCTGC10.12254901960784313No Hit
CGGAAACAAAACAGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCT10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAACTCTCAGC10.12254901960784313No Hit
CGGATACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAAACTGGAGACCAAGTCTCTGCT10.12254901960784313No Hit
CGAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGCTA10.12254901960784313No Hit
CGGAAACAACAACTGCAGCATGGCAGGATAGCAACTGGAGACCAAGTCTC10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGACTCTGA10.12254901960784313No Hit
CGGAAACAACAACTCCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CGTTAGTGACAAGAGGGACATCTGAGAAGAGGAAGAACTGGAGACCAAGT10.12254901960784313No Hit
CGGAAACAACAACTGCTGAGAAGAGAAAGAACTGGAGACCAAGTCTATCC10.12254901960784313No Hit
CGGAAACAGCAACTGCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit
CTGAAACAACAACTTCTGAGAAGAGGAAGAACTGGAGACCAAGTCTCTGC10.12254901960784313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)