FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28521
Total Bases4.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC864830.321517478349286No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC472516.566740296623543No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT412314.456014866238911No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT324911.391606184916379No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC24708.660285403737596No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG10753.7691525542582656No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG3021.0588689036148802No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT810.2840012622278321No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA750.26296413169243715No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG750.26296413169243715No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT620.21738368219908136No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG590.2068651169313839No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCCTATCATTCAC480.16829704428315978No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA450.1577784790154623No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC440.15427229059289646No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC430.15076610217033062No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC430.15076610217033062No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA400.14024753690263314No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA390.1367413484800673No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT380.1332351600575015No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT350.12271659478980401No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA340.11921040636723819No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC330.11570421794467234No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC290.10167946425440903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)