FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22506
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA477721.225450990846888No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC440319.563671909712966No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC357915.902426019728072No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT324714.427263840753577No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT312413.880742913000976No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC5122.274948902514885No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT620.27548209366391185No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC500.2221629787612192No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT450.19994668088509732No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA430.19106016173464854No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT410.18217364258419977No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA380.1688438638585266No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC380.1688438638585266No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT340.15107082555762907No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA280.12441126810628278No Hit
CGTTAGTGACAAGAGGGCCATCTCGCGATTTCTCGAGTCCAACCCTGTGA280.12441126810628278No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT270.11996800853105838No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT270.11996800853105838No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.11552474895583399No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT230.10219497023016084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)