FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5307
Total Bases769.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT262349.42528735632184No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC125223.591482947051066No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC58911.098549086112682No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG1122.1104201997361973No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT240.4522328999434709No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT220.4145468249481816No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT160.3014885999623139No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT150.2826455624646693No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT140.26380252496702467No Hit
TGGCTCATCTTCAAACGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT130.24495948746938007No Hit
TGGTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT130.24495948746938007No Hit
TGGCTCATTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT130.24495948746938007No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT120.22611644997173544No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT100.1884303749764462No Hit
TGGCTCATCTTCAAACCGTGGGCAACTCTGGCCGGCCTCTGCTGAATGAC100.1884303749764462No Hit
TGGCTCATCTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT90.1695873374788016No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG90.1695873374788016No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC80.15074429998115696No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGCGATGAT80.15074429998115696No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT70.13190126248351233No Hit
TGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT70.13190126248351233No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGGTGAT70.13190126248351233No Hit
TGGCTCTCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT60.11305822498586772No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCCGTGATGAT60.11305822498586772No Hit
TGGCCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT60.11305822498586772No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGCCCAACCCTGTGATGAT60.11305822498586772No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGT60.11305822498586772No Hit
AGACCCCTGGACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT60.11305822498586772No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAGCCCTGTGATGAT60.11305822498586772No Hit
TGGCTCACTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT60.11305822498586772No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCAGTGATGAT60.11305822498586772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)