FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12154
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC655353.9164061214415No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT370630.492019088365968No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC2992.46009544182985No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC1190.9791015303603752No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA640.5265756129669245No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT560.46075366134605894No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT380.3126542701991114No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT350.2879710383412868No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT330.2715155504360704No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC300.24683231857824586No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT270.22214908672042125No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC270.22214908672042125No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.20569359881520485No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC210.1727826230047721No Hit
AGACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT200.1645548790521639No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC190.1563271350995557No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC190.1563271350995557No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.1563271350995557No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT180.1480993911469475No Hit
AGCAAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT160.13164390324173111No Hit
AGCACAGGACTCTTCTTTGGCATCAATCATCACAGGGTTGGACTACAAAA160.13164390324173111No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.12341615928912293No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC150.12341615928912293No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGCCCTGCT140.11518841533651474No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAGGCTCCCACCCAGCGC130.10696067138390654No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC130.10696067138390654No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT130.10696067138390654No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT130.10696067138390654No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAACTCCCACCCAGCGC130.10696067138390654No Hit
CGTTAGTGACAAGAGGGACATCTGAGAAGAGGAAGTTGCCAAGACCAAGT130.10696067138390654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)