FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22011
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC532424.18790604697651No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC461820.980418881468356No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT327014.856208259506609No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC268612.202989414383717No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT19598.900095406842032No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG6382.898550724637681No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG3201.453818545272818No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT900.4088864658579801No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT800.3634546363182045No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTGCCTACGGGCTGC630.28622052610058607No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG530.24078869656081048No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT400.18172731815910226No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA380.17264095225114715No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTGCCTACGGGCTGC380.17264095225114715No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA340.15446822043523695No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG340.15446822043523695No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC330.14992503748125938No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC330.14992503748125938No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGTAGCTCCAGCTTATCATTCAC300.1362954886193267No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA290.13175230566534915No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT280.12720912271137158No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT270.12266593975739402No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA260.11812275680341648No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT260.11812275680341648No Hit
CTTCCTCTTCTCAGATGTCCCTCTTGTCACTAACGCAGTCATGTAGAACC250.11357957384943891No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT250.11357957384943891No Hit
GCTATCCTCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA240.10903639089546137No Hit
AGACCCCTGGAATGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT230.10449320794148381No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA230.10449320794148381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)