FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12344
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC456436.97342838626053No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT303724.60304601425794No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC253320.5200907323396No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT2151.7417368762151653No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG1341.085547634478289No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG1150.9316267012313674No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT760.6156837329876863No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT710.5751782242384964No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT510.4131561892417369No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC360.2916396629941672No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT320.2592352559948153No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG320.2592352559948153No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG250.20252754374594945No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT240.19442644199611148No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT230.18632534024627348No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT220.1782242384964355No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT220.1782242384964355No Hit
AGCCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT200.16202203499675957No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT180.1458198314970836No Hit
TGGCTCATCTTCAAACGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT170.1377187297472456No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT160.12961762799740764No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCCGC150.12151652624756966No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAGGGGTACCTACGGGCTGC140.11341542449773169No Hit
AGACCCCTGGAACTGCTACATGTGTGGGCACAAGGGTACCTACGGGCTGC140.11341542449773169No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCAGTGATGAT140.11341542449773169No Hit
TGGCTCATCTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT130.10531432274789371No Hit
AGACCCCGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT130.10531432274789371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)