FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27536
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA913633.17838466008135No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC616522.388872748402093No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT417715.169233004067403No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT336112.205839628123185No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC8723.1667635095874487No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC5361.946542707728065No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT990.3595293434049971No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT730.26510749564206854No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT660.23968622893666475No Hit
CGTTAGTGACAAGAGGGCCATCTCGCGATTTCTCGAGTCCAACCCTGTGA590.2142649622312609No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA510.18521208599651365No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT440.15979081929110983No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT420.152527600232423No Hit
CGTTAGTGAAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA420.152527600232423No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT420.152527600232423No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT390.1416327716443928No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT380.1380011621150494No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC380.1380011621150494No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA370.134369552585706No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT320.11621150493898895No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGG290.10531667635095875No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT280.10168506682161534No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGCCCAACCCTGTGA280.10168506682161534No Hit
CGTTAGTGCCAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA280.10168506682161534No Hit
AGCACAGGACTCTTCTTTGGCATCAATCATCACAGGGTTGGACTACAAAA280.10168506682161534No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT280.10168506682161534No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT280.10168506682161534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)