FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13430
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC526339.18838421444527No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC321823.961280714817573No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT231717.2524199553239No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAC3942.9337304542069993No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG1861.3849590469099033No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT890.6626954579300074No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT710.5286671630677587No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAAGGTACCTACGGGCTGC480.3574087862993298No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT470.34996276991809383No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT390.2903946388682055No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG380.2829486224869695No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAAGGTACCTACGGGCTGC380.2829486224869695No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC350.2606105733432614No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG300.22338049143708116No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT260.193596425912137No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT240.1787043931496649No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT220.16381236038719285No Hit
AGACCCCTGGAACTGCTCCATGTGCGGGCACAAGGGTACCTACGGGCTGC190.14147431124348475No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG190.14147431124348475No Hit
AGACCCCTGGAACTGCTACATGTGTGGGCACAAGGGTACCTACGGGCTGC180.13402829486224868No Hit
AGACCCCGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT180.13402829486224868No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT180.13402829486224868No Hit
AGCCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT170.12658227848101267No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCCGC170.12658227848101267No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT170.12658227848101267No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT150.11169024571854058No Hit
AGACCCCTGGAATGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT150.11169024571854058No Hit
AGACCCCTGGACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT140.10424422933730454No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAGGGGTACCTACGGGCTGC140.10424422933730454No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGGTGAT140.10424422933730454No Hit
AGACCCCTGGAACTGTTACATGTGCGGGCACAAGGGTACCTACGGGCTGC140.10424422933730454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)