FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27247
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA848531.141043050611078No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC496718.229529856497965No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC432415.86963702425955No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT327112.00499137519727No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT21988.066943149704555No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC6522.3929239916321063No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAATACTTTTAAAGGTCCTGCT940.34499210922303375No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT850.31196094982933903No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC790.2899401769002092No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA630.231218115755863No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT600.22020772929129814No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA590.2165376004697765No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA570.20919734282673322No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC560.20552721400521157No Hit
CGTTAGTGACAAGAGGGCCATCTCGCGATTTCTCGAGTCCAACCCTGTGA500.18350644107608177No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT380.13946489521782215No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT340.1247843799317356No Hit
CGTTAGTGAAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA340.1247843799317356No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT340.1247843799317356No Hit
CGTTAGTGAGAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA340.1247843799317356No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT330.12111425111021397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)