FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008141989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008141989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20345
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC610630.012288031457363No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC421920.737281887441632No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT337116.569181617104938No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC222910.95600884738265No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT10185.003686409437208No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG5762.831162447775866No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG830.40796264438436963No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT690.3391496682231506No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT610.29982796755959695No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG560.2752519046448759No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG550.27033669206193167No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA480.2359302039813222No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA450.22118456623248955No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT450.22118456623248955No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC330.162202015237159No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA320.1572868026542148No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC310.15237159007127057No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTATGGGCTGC280.13762595232243796No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT260.12779552715654952No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT260.12779552715654952No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT240.1179651019906611No Hit
GCTATCCTGCCTGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA220.10813467682477267No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT210.10321946424182846No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA210.10321946424182846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)