FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18797
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC606832.28174708730116No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA377920.104271958291218No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC323817.226153109538757No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT280914.943874022450391No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC4092.175879129648348No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT3171.6864393254242698No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT430.22875990849603658No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT400.21279991488003402No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT390.20747991700803317No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT300.15959993616002555No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC280.14895994041602384No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCAC260.13831994467202213No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC260.13831994467202213No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT240.12767994892802043No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT240.12767994892802043No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT240.12767994892802043No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT230.12235995105601959No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA220.11703995318401872No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT220.11703995318401872No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT210.11171995531201787No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA200.10639995744001701No Hit
CGTTAGTGACAAGAGGGACATCTGAGAAGAGGAAGTTGCCAAGACCAAGT200.10639995744001701No Hit
CGTTAGTGACAAGAGGGCCATCTCGCGATTTCTCGAGTCCAACCCTGTGA190.10107995956801619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)