FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27945
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC874831.30434782608696No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC511718.31096797280372No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT327311.712292002147075No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC307411.000178922884237No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT304410.892825192342102No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG6762.4190373948828054No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG3681.316872427983539No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT810.2898550724637681No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA780.2791196994095545No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT670.23975666487743783No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA560.20039363034532118No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG530.18965825729110752No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT510.18250134192163178No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC460.1646090534979424No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG400.1431383073895151No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC400.1431383073895151No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA400.1431383073895151No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA390.13955984970477725No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT370.13240293433530148No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCGGCTCCAGCTTATCATTCAC330.11808910359634997No Hit
GCTATCCTGCCTGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA310.11093218822687421No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT300.10735373054213634No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT300.10735373054213634No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA290.10377527285739846No Hit
GCTATCCTGCCATGCTCCAGACACTCCCGCAGCTCCAGCTTATCATTCAC290.10377527285739846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)