FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5509
Total Bases798.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT209337.99237611181703No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC124422.58123071337811No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC83815.211472136503904No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT5479.929206752586676No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG340.6171719005264112No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT150.2722817208204756No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT150.2722817208204756No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT140.25412960609911056No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT130.23597749137774549No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT130.23597749137774549No Hit
TGGTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT120.2178253766563805No Hit
TGGCTCATCTTCAAACGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT100.1815211472136504No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT90.16336903249228535No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGCCCAACCCTGTGATGAT90.16336903249228535No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTGGTCCAACCCTGTGATGAT90.16336903249228535No Hit
TGGCTCATCTTCAAACCGTTTCCTGTTTTGTAGTCCAACCCTGTGATGAT90.16336903249228535No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC80.14521691777092033No Hit
TGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC80.14521691777092033No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGGTGAT80.14521691777092033No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT70.12706480304955528No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT70.12706480304955528No Hit
AACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT70.12706480304955528No Hit
AGCCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT60.10891268832819025No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGAGATGAT60.10891268832819025No Hit
TGGCTCATCTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT60.10891268832819025No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC60.10891268832819025No Hit
AGCCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT60.10891268832819025No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAGCCCTGTGATGAT60.10891268832819025No Hit
TGGCTCATTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT60.10891268832819025No Hit
AGACCCCTGGAACTGCTCCATGTGCGGGCACAAGGGTACCTACGGGCTGC60.10891268832819025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)