FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30531
Total Bases4.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA826327.064295306409882No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC636220.83783695260555No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT618620.261373685762013No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT336811.031410697324032No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC19266.308342340571878No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC6061.9848678392453571No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT950.3111591497166814No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT660.21617372506632604No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC660.21617372506632604No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT630.2063476466542203No Hit
CGTTAGTGACAAGAGGGCCATCTCGCGATTTCTCGAGTCCAACCCTGTGA600.19652156824211459No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA590.19324620877141266No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC580.18997084930071076No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT560.18342013035930693No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT490.16049261406439358No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT450.14739117618158593No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA430.1408404572401821No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT410.1342897382987783No Hit

[OK]Adapter Content

Adapter graph