FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24847
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC790131.798607477763912No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC496519.98229162474343No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC330113.285306073167789No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT287811.58288727009297No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT13145.2883647925302855No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG8303.3404435143075624No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG1600.6439409184207349No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT850.3420936129110154No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG730.2937980440294603No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT560.2253793214472572No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA540.21733005996699803No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA520.20928079848673886No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT500.20123153700647967No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG420.1690344910854429No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT360.14488670664466535No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT330.1328128144242766No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA320.128788183684147No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA310.12476355294401739No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC310.12476355294401739No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC300.12073892220388778No Hit
CTTCCTCTTCTCAGATGTCCCTCTTGTCACTAACGCAGTCATGTAGAACC290.1167142914637582No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT280.1126896607236286No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT270.10866502998349901No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGT250.10061576850323983No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA250.10061576850323983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)