FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22415
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC858738.30916796787866No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC411018.33593575730538No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT317614.169083203212136No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC287812.83961632835155No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG3051.3606959625250947No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT1780.7941110863261208No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT760.3390586660718269No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC680.3033682801695293No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA660.29444568369395496No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT540.24091010484050857No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA530.23644880660272138No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA500.2230649118893598No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT490.2186036136515726No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG480.21414231541378542No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT420.18737452598706225No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG420.18737452598706225No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC330.14722284184697748No Hit
GCTATCCTGCCTGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA320.14276154360919027No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA310.13830024537140306No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTACCATTCAC300.13383894713361588No Hit
GCTATCCTGCCATGCTCCAGACACTCCCGCAGCTCCAGCTTATCATTCAC300.13383894713361588No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT280.12491635065804149No Hit
GCTATCCTGCCATGCTCCAGGCACTCCTGCAGCTCCAGCTTATCATTCAC280.12491635065804149No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA280.12491635065804149No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATCCAC270.12045505242025428No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT270.12045505242025428No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCGGCTTATCATTCAC250.1115324559446799No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA250.1115324559446799No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCCCCAGCTTATCATTCAC240.10707115770689271No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCGGCTCCAGCTTATCATTCAC230.10260985946910552No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT230.10260985946910552No Hit
GCTACCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA230.10260985946910552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)