FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23812
Total Bases3.4 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA519821.82932974970603No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC490620.603057282042666No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC446118.734251637829665No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT403016.92423987905258No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT15796.63111036452209No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC6412.6919200403158072No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCCA750.3149672434066857No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC620.2603729212161935No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT500.20997816227112379No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT470.19737947253485638No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT430.18058121955316647No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC410.17218209306232152No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT400.16798252981689904No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA390.16378296657147656No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT390.16378296657147656No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA340.14278515034436418No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT330.13858558709894173No Hit
AGCACAGGACTCTTCTTTGGCATCAATCATCACAGGGTTGGACTACAAAA290.12178733411725182No Hit
CGTTAGTGACAAGAGGGACATCTGAGAAGAGGAAGTTGCCAAGACCAAGT290.12178733411725182No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT260.10918864438098437No Hit
AGACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT260.10918864438098437No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.10918864438098437No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.10498908113556189No Hit
CGTTAGTGAAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA240.10078951789013943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)