FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16803
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC613136.48753198833541No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC439426.150092245432365No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT228313.586859489376895No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT6393.8028923406534547No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG5673.3743974290305303No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG3191.8984705112182347No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT1000.5951318216985062No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT820.4880080937927751No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT700.41659227518895436No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT630.3749330476700589No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC590.35112777480211865No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG470.2797119561982979No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG390.23210141046241742No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT320.19044218294352197No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT310.18449086472653692No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCCGC300.17853954650955187No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC250.14878295542462655No Hit
AACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT240.14283163720764147No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC210.12497768255668631No Hit
AGCCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT210.12497768255668631No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC200.11902636433970125No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTAC200.11902636433970125No Hit
AGACCCCTGGAACTGCTACATGTGCGGACACAAGGGTACCTACGGGCTGC190.11307504612271617No Hit
AGACCCCTGGAACTGTTACATGTGCGGGCACAAGGGTACCTACGGGCTGC190.11307504612271617No Hit
AGACCCCTGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT190.11307504612271617No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACGAGGGTACCTACGGGCTGC180.10712372790573112No Hit
AGACCCCGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT180.10712372790573112No Hit
AGACCCCTGGAACTGCTACCTGTGCGGACACAAGGGTACCTACGGGCTGC180.10712372790573112No Hit
AGCCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT180.10712372790573112No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC180.10712372790573112No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC180.10712372790573112No Hit
CTTCCTCTTCTCAGATGTCCCTCTTGTCACTAACGCAGTCATGTAGAACC170.10117240968874606No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACGAGGGTACCTACGGGCTGC170.10117240968874606No Hit
AGACCCCTGGAACTGCTACATGTGTGGGCACAAGGGTACCTACGGGCTGC170.10117240968874606No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT170.10117240968874606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)