FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24969
Total Bases3.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC875935.079498578237015No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA618724.77872561976851No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT311112.459449717649886No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT19677.877768432856742No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGGTACAAAATAGCC10744.301333653730626No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC6672.671312427409988No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT730.2923625295366254No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC670.2683327325884096No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT660.26432776643037365No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA580.23228803716608595No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT540.2162681725339421No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC510.20425327405983418No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT500.20024830790179826No Hit
CGTTAGTGACAAGAGGGCCATCTCGCGATTTCTCGAGTCCAACCCTGTGA470.18823340942769035No Hit
CGTTAGTGAAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA430.17221354479554646No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT430.17221354479554646No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT420.16820857863751051No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA400.16019864632143857No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT360.14417878168929474No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT330.13216388321518682No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT290.11614401858304298No Hit
ACAACAGGTCAGATGCAGGCCCAGTGGTGTCCCAAGCTCCCACCCAGCGC280.112139052425007No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT280.112139052425007No Hit
CGTTAGTGACAAGAGGGACATCTGAGAAGAGGAAGTTGCCAAGACCAAGT280.112139052425007No Hit
ACAACAGGTCAGATGCAGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT270.10813408626697105No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT270.10813408626697105No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGCGTCCCAAGCTCCCACCCAGCGC270.10813408626697105No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCCCCCACCCAGCGC260.10412912010893509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)