FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13568
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC1125282.93042452830188No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC4593.3829599056603774No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT1000.7370283018867925No Hit
CGGAAACAACAACTGCTGCGGCGGCGAGAGGACTGGCCCTCCCGGCTCTA930.685436320754717No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT860.6338443396226415No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT640.4716981132075472No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT560.41273584905660377No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT480.3537735849056604No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT430.31692216981132076No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT420.3095518867924528No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC410.30218160377358494No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT370.2727004716981132No Hit
CGGAAACAACAACTGCTGCGAAGAGGAAGTTGCCAAGACCAAGTCTCTGC310.22847877358490565No Hit
ACAACAGGTCAGATGCAGGTCCATCGGTGTCCCAAGCTCCCACCCAGCGC290.2137382075471698No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC290.2137382075471698No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCCCCCACCCAGCGC290.2137382075471698No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC270.19899764150943397No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.18425707547169812No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT250.18425707547169812No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCAC240.1768867924528302No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCGAGCTCCCACCCAGCGC230.16951650943396226No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC220.16214622641509435No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC220.16214622641509435No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT210.1547759433962264No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGTTCCCACCCAGCGC210.1547759433962264No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCGCCCAGCGC190.14003537735849056No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.14003537735849056No Hit
ACAACAGGTCAGATGCAGGCCCATCGATGTCCCAAGCTCCCACCCAGCGC180.13266509433962265No Hit
ACAACAGGTCAGATGTAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC170.1252948113207547No Hit
CGTTAGTGACAAGAGGGACATCTGAGAAGAGGAAGTTGCCAAGACCAAGT170.1252948113207547No Hit
ACAACAGGTCAGATGCAGGCCAATCGGTGTCCCAAGCTCCCACCCAGCGC160.1179245283018868No Hit
ACAACAGGTCAGATGCGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT160.1179245283018868No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAGGCTCCCACCCAGCGC150.11055424528301887No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCGGCGC150.11055424528301887No Hit
ACAACAGGTCAGATGCAGGCCCATCAGTGTCCCAAGCTCCCACCCAGCGC140.10318396226415094No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCTCAAGCTCCCACCCAGCGC140.10318396226415094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)