FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13568
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC697351.39298349056604No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC400829.54009433962264No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG4603.3903301886792456No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT1080.7959905660377359No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT1010.7443985849056604No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG930.685436320754717No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT810.5969929245283019No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC600.44221698113207547No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT560.41273584905660377No Hit
GACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT460.33903301886792453No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG400.294811320754717No Hit
CTTCCTCTTCGCAGCAGTTGTTGTTTCCGCAGTCATGTAGAACCATGTCG310.22847877358490565No Hit
AGCCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT270.19899764150943397No Hit
AGACCCCTGGAACTGCTACATGTGTGGGCACAAGGGTACCTACGGGCTGC260.19162735849056603No Hit
AGACCCCTGGAACTGTTACATGTGCGGGCACAAGGGTACCTACGGGCTGC260.19162735849056603No Hit
AGACCCCGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT240.1768867924528302No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT240.1768867924528302No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT230.16951650943396226No Hit
AGACCCCTGGAACTGCTACCGTGCGGGCACAAGGGTACCTACGGGCTGCT230.16951650943396226No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGACTGC210.1547759433962264No Hit
AGACCCCTGGAACTGCTACATGTGCGGACACAAGGGTACCTACGGGCTGC200.1474056603773585No Hit
AACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT200.1474056603773585No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCCACGGGCTGC190.14003537735849056No Hit
AGACCCCTGGACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT190.14003537735849056No Hit
CTTCCTCTTCTCAGATGTCCCTCTTGTCACTAACGCAGTCATGTAGAACC170.1252948113207547No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGATACCTACGGGCTGC170.1252948113207547No Hit
AGACCCCTGGAATGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT170.1252948113207547No Hit
AGACCCCTGGACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT160.1179245283018868No Hit
AGACCCCTGGAACTGCTCCATGTGCGGGCACAAGGGTACCTACGGGCTGC160.1179245283018868No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTAC160.1179245283018868No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC150.11055424528301887No Hit
AGACCCCTGGAACTGCTACATGCGCGGGCACAAGGGTACCTACGGGCTGC140.10318396226415094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)