FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23648
Total Bases3.4 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA678628.695872801082544No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC459119.41390392422192No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT385416.297361299052774No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT293812.423883626522327No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC21268.990189445196211No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC2911.2305480378890392No Hit
CGTTAGTGACAAGGGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA650.27486468200270636No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA560.2368064952638701No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT510.21566305818673887No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT510.21566305818673887No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT490.20720568335588635No Hit
CGTTAGTGACAAAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT490.20720568335588635No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT460.19451962110960758No Hit
CGTTAGTGACAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT450.19029093369418135No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT420.17760487144790257No Hit
CGGAAACAACAACGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT420.17760487144790257No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA350.1480040595399188No Hit
CGGAAACAACAACTCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT330.13954668470906628No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT330.13954668470906628No Hit
CGTTAGTGACAAGAGGGACTCTCGCGATTTCTCGAGTCCAACCCTGTGAT320.13531799729364005No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT300.12686062246278756No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT280.11840324763193505No Hit
CGGAAACAACAATGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT270.11417456021650879No Hit
CGGAAACAAAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT270.11417456021650879No Hit
CGTTATGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT260.10994587280108255No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.10994587280108255No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGG260.10994587280108255No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT260.10994587280108255No Hit
CGTTGGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA240.10148849797023003No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT240.10148849797023003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)