FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12113
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC639752.811029472467595No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT413634.145133327829605No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT720.594402707834558No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT700.5778915215058202No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC500.412779658218443No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT480.3962684718897053No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT370.30545694708164783No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT340.28069016758854126No Hit
ACAACGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT310.25592338809543463No Hit
AGCAAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT290.23941220176669692No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT290.23941220176669692No Hit
ACAACAGGTCAATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT280.23115660860232806No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.21464542227359035No Hit
ACAACAGGTCAGATGCAGGTCCATCGGTGTCCCAAGCTCCCACCCAGCGC260.21464542227359035No Hit
AGCACAGGACTTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT240.19813423594485266No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT230.18987864278048378No Hit
AGCACAGGATCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT200.1651118632873772No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.15685627012300835No Hit
ACAACAGGTCAGATGCAGGCCCATCGGCGTCCCAAGCTCCCACCCAGCGC190.15685627012300835No Hit
ACAACAGGTCAGATGCAGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT190.15685627012300835No Hit
AGACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT180.1486006769586395No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGCCCCAAGCTCCCACCCAGCGC170.14034508379427063No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGCCCTGCT150.12383389746553289No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGGGTACTTTTAAAGGTCCTGCT150.12383389746553289No Hit
AGCACGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT140.11557830430116403No Hit
ACACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT140.11557830430116403No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGTGC130.10732271113679517No Hit
ACAACAGGTCAGATGCGGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC130.10732271113679517No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAACTCCCACCCAGCGC130.10732271113679517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)