FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27788
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA1079738.85490139628617No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC811529.20325320282136No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT22308.025046782783935No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT16706.009788397869584No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC7272.61623722470131No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA1270.45703181229307616No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC1180.42464373110695264No Hit
CGTTAGTGACAAGGGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA1130.40665035267021743No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA1110.3994530012955233No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT830.2986900820498057No Hit
CGTTAGTGACAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT800.2878940549877645No Hit
CGTTAGTGACAAAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT760.2734993522383763No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT710.255505973801641No Hit
CGTTGGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA530.19072981142939396No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT470.16913775730531164No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT460.16553908161796457No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT450.16194040593061754No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT440.15834173024327047No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGCCCAACCCTGTGA410.1475457031812293No Hit
CGTTATGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT390.1403483518065352No Hit
ACAACAGGTCGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT380.13674967611918815No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT380.13674967611918815No Hit
CGTTAGTGACAAGAGGGACTCTCGCGATTTCTCGAGTCCAACCCTGTGAT380.13674967611918815No Hit
CGGAAACAACAACTCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT370.13315100043184108No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCCGTGA370.13315100043184108No Hit
ACAACAGGTCAGTGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT360.12955232474449402No Hit
ACAACAGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT350.12595364905714695No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCCCGAGTCCAACCCTGTGA350.12595364905714695No Hit
CGTTAGTGACAAGAGGGACATCTCGCGACTTCTCGAGTCCAACCCTGTGA340.1223549733697999No Hit
CGTTGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT330.11875629768245287No Hit
GTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT330.11875629768245287No Hit
CTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT330.11875629768245287No Hit
CGTTAGTGCAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT320.1151576219951058No Hit
CGTTAGTGACAAGAGGGCATCTCGCGATTTCTCGAGTCCAACCCTGTGAT310.11155894630775876No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGG310.11155894630775876No Hit
ACAACAGGTAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.10796027062041169No Hit
ACAACAGGCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.10796027062041169No Hit
CGTTAGTGACAAGAGGGAATCTCGCGATTTCTCGAGTCCAACCCTGTGAT280.10076291924571758No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAGCCCTGTGA280.10076291924571758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)