FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25933
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA844232.55311764932711No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC406815.686576948289824No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT333012.840782015192998No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT326012.570855666525278No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC281210.843327035051864No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA1280.49357960899240355No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA1230.4742991555161377No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC1120.43188215786835304No Hit
CGTTAGTGACAAGGGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA850.327767709096518No Hit
CGTTAGTGACAAAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT720.277638530058227No Hit
CGTTAGTGACAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT670.2583580765819612No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT580.22365326032468283No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT550.21208498823892338No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT440.1696679905911387No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT430.16581189989588557No Hit
CGGAAACAACAACTCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT420.16195580920063238No Hit
CGTTGGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA400.1542436278101261No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT390.15038753711487293No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT380.1465314464196198No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT370.14267535572436663No Hit
CGTTAGTGACAAGAGGGACTCTCGCGATTTCTCGAGTCCAACCCTGTGAT370.14267535572436663No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGG360.1388192650291135No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT330.12725099294335404No Hit
CGGAAACAAAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT310.11953881155284773No Hit
CGGAAACAACAACGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT310.11953881155284773No Hit
CGTTATGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT300.11568272085759457No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT300.11568272085759457No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT300.11568272085759457No Hit
GTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT300.11568272085759457No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT300.11568272085759457No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT290.11182663016234141No Hit
CGTTAGTGACAAGAGGGACATCTCGCGACTTCTCGAGTCCAACCCTGTGA280.10797053946708826No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGCGA260.10025835807658195No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT260.10025835807658195No Hit
CTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT260.10025835807658195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)