FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20989
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA684832.62661394063557No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC673732.0977654962123No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT339816.189432559912333No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT9504.526180380199152No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC1360.6479584544285102No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT720.3430368288150936No Hit
CGTTAGTGACAAGGGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA680.3239792272142551No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA660.3144504264138358No Hit
CGTTAGTGACAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT630.3001572252132069No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT620.2953928248129973No Hit
CGTTAGTGACAAAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT590.2810996236123684No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA500.2382200200104817No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT460.21916241840964312No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC390.18581161560817572No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT390.18581161560817572No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT380.18104721520796607No Hit
CGTTAGTGACAAGAGGGACTCTCGCGATTTCTCGAGTCCAACCCTGTGAT360.1715184144075468No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT330.1572252132069179No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT320.15246081280670828No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT320.15246081280670828No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT290.13816761160607938No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT260.12387441040545048No Hit
CGTTGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT260.12387441040545048No Hit
CGTTATGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT240.11434560960503119No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGCGA240.11434560960503119No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT240.11434560960503119No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAGCCCTGTGA230.10958120920482156No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT230.10958120920482156No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGCCCAACCCTGTGA220.10481680880461194No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCCCGAGTCCAACCCTGTGA210.10005240840440231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)