FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12683
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC579945.722620831033666No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT295623.30678861468107No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC227817.96105022471024No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT1020.8042261294646377No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT440.3469210754553339No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC290.22865252700465188No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG290.22865252700465188No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT270.21288338721122763No Hit
AGACCCCGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT240.18922967752109124No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT240.18922967752109124No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT240.18922967752109124No Hit
AGACCCCTGGACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT240.18922967752109124No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT200.1576913979342427No Hit
TGGCTCATCTTCAAACGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT200.1576913979342427No Hit
AGACCCCTGGAATGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT200.1576913979342427No Hit
AGCCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT190.14980682803753056No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTAC170.13403768824410628No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTATCTACGGGCTGC170.13403768824410628No Hit
AGACCCCTGGAACTGCTACATGTGTGGGCACAAGGGTACCTACGGGCTGC160.12615311834739415No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCCGC160.12615311834739415No Hit
TGGCTATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT150.11826854845068202No Hit
GACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT150.11826854845068202No Hit
TGGCTCATTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT140.11038397855396988No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGT130.10249940865725775No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG130.10249940865725775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)