FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14001
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC633845.268195128919366No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT489134.93321905578173No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC2902.0712806228126563No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG2411.7213056210270694No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC1621.1570602099850011No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC710.5071066352403399No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA610.4356831654881794No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA500.3571173487608028No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC390.2785515320334262No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC370.26426683808299406No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA360.257124491107778No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC350.24998214413256195No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC300.2142704092564817No Hit
CTTCCTCTTCTCAGCTGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC290.2071280622812656No Hit
CTTCCTCTTCTCACTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC270.19284336833083351No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA270.19284336833083351No Hit
GCTATCCTGCCATGCTCCAGACACTCCCGCAGCTCCAGCTTATCATTCAC260.18570102135561745No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC250.1785586743804014No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA250.1785586743804014No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA250.1785586743804014No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCGGCTTATCATTCAC240.17141632740518534No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC240.17141632740518534No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTACCATTCAC220.15713163345475323No Hit
CTTCCTCTTCTCAGCTGAGACAGGTGGGTAAACCTTTGGAGGGTCAAATT220.15713163345475323No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG210.14998928647953716No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA200.14284693950432112No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCGGCTCCAGCTTATCATTCAC190.13570459252910508No Hit
GCTATCCTGCCATGCTCCAGACACCCCTGCAGCTCCAGCTTATCATTCAC180.128562245553889No Hit
GCTATCCTGCCATGCTCCAGGCACTCCTGCAGCTCCAGCTTATCATTCAC170.12141989857867294No Hit
GCTACCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA170.12141989857867294No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATCCAC160.1142775516034569No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCGC160.1142775516034569No Hit
CTTCCTCTTCCCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT160.1142775516034569No Hit
CTTCCTCTTCTCAGCTGGGACGGGTGGGTAAACCTTTGGAGGGTCAAATT160.1142775516034569No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAGCCTTTGGAGGGTCAAATT150.10713520462824085No Hit
GCTATCCTGCCATGCTCCAGACGCTCCTGCAGCTCCAGCTTATCATTCAC150.10713520462824085No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAACTCCAGCTTATCATTCAC150.10713520462824085No Hit
CTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC150.10713520462824085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)