FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26230
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC836231.8795272588639No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT670825.573770491803277No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT333412.710636675562334No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC306111.669843690430804No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC10303.926801372474266No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG1420.5413648494090736No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC1030.3926801372474266No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA760.2897445672893633No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA540.20587113991612657No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA520.19824628288219598No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG520.19824628288219598No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC480.18299656881433474No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT440.16774685474647352No Hit
CTTCCTCTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC430.16393442622950818No Hit
CTTCCTCTTCTCGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC400.15249714067861228No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGCCAAATT390.14868471216164697No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC390.14868471216164697No Hit
CTTCCTCTTCTCAGCTGGGACGGGTGGGTAAACCTTTGGAGGGTCAAATT390.14868471216164697No Hit
CTTCCTCTTCTCACTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC380.14487228364468166No Hit
CTTCCTCTTCTCAGCTGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC360.13724742661075104No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA350.13343499809378576No Hit
GCTATCCTGCCATGCTCCAGACACTCCCGCAGCTCCAGCTTATCATTCAC350.13343499809378576No Hit
CTTCCTCTTCTCAGCTGGGCAGGTGGGTAAACCTTTGGAGGGTCAAATTC350.13343499809378576No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA310.11818528402592451No Hit
CTTCCTTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC300.1143728555089592No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTACCATTCAC300.1143728555089592No Hit
CTTCCTCTTTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC290.11056042699199391No Hit
GCTATCCTGCCATGCTCCAGGCACTCCTGCAGCTCCAGCTTATCATTCAC270.10293556995806329No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCCCCAGCTTATCATTCAC270.10293556995806329No Hit
CTTCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC270.10293556995806329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)