FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29566
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA757325.613880809037408No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT657422.234999661773656No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC634121.44693228708652No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT392313.268619360075762No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGGCCGTTGGCATCC7612.5739024555232364No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC3971.3427585740377461No Hit
CGGAAACAACACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT1180.399107082459582No Hit
CGGAAACACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT980.3314618142460935No Hit
CGGAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT890.3010214435500237No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA690.2333761753365352No Hit
CGTTAGTGACAAGGGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA650.21984712169383752No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT640.2164648582831631No Hit
CGGAAACAACAACTCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT630.21308259487248868No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT600.20293580464046543No Hit
CGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT530.17925996076574444No Hit
CGTTAGTGACAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT520.17587769735507003No Hit
CGGAAACAACAATGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT500.16911317053372116No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCTTGTGA470.15896638030169788No Hit
CGTTAGTGACAAAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT450.15220185348034906No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT440.14881959006967463No Hit
CGGAAACAACAACGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT430.1454373266590002No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT410.13867279983765135No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT410.13867279983765135No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA370.12514374619495366No Hit
CGGAAACAAAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTT370.12514374619495366No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT360.12176148278427924No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT310.10485016573090712No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT310.10485016573090712No Hit
CGTTAGTGCAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT300.10146790232023271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)