FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18948
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC639533.75026388009289No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT379620.033776651889383No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT331517.495250158328055No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC215011.346843994089086No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG4972.6229681232847795No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG3011.588558159172472No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT830.4380409541904159No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT530.27971289845894026No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC300.15832805573147563No Hit
CTTCCTCTTCTCAGCTGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG300.15832805573147563No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT280.1477728520160439No Hit
AGACCCCTGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT260.1372176483006122No Hit
TGGTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT240.1266624445851805No Hit
CTTCCTCTTCTCAGTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC230.12138484272746465No Hit
AGCCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT220.11610724086974879No Hit
AACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT220.11610724086974879No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCCGC210.11082963901203294No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGACTGC200.10555203715431707No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC190.10027443529660121No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT190.10027443529660121No Hit
AGACCCCTGGAACTGCTACATGTGTGGGCACAAGGGTACCTACGGGCTGC190.10027443529660121No Hit
CTTCCTCTTCTCAGCTGAGACAGGTGGGTAAACCTTTGGAGGGTCAAATT190.10027443529660121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)