FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29642
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC1063335.87139869104649No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC540218.224141420956748No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT322510.879832669860333No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT322410.876459078334795No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC25958.754470008771339No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG2991.0087038661358882No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG2680.9041225288442076No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC1100.37109506780918966No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA880.29687605424735175No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT790.26651373051750893No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA640.21590985763443762No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA610.20578908305782334No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA540.18217394237905674No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT490.16530598475136632No Hit
GCTATCCTGCCATGCTCCAGACACTCCCGCAGCTCCAGCTTATCATTCAC390.13157006949598543No Hit
AGACCCCTGGAACTGCTACATGTGTGGGCACAAGGGTACCTACGGGCTGC380.12819647797044734No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA350.11807570339383308No Hit
GCTACCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA340.11470211186829497No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATCCAC340.11470211186829497No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA340.11470211186829497No Hit
AGACCCCTGGAACTGCTACGTGTGCGGGCACAAGGGTACCTACGGGCTGC320.10795492881721881No Hit
CTTCCTCTTCTAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATTC310.10458133729168073No Hit
TGGCTCATCTTAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT300.10120774576614265No Hit
GCTATCCGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA300.10120774576614265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)