FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436
Total Bases62.9 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT25458.256880733944946No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC9020.642201834862387No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC132.981651376146789No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTAAAAGGTCCTGCT40.9174311926605505No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACCTTTAAAGGTCCTGCT30.6880733944954129No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT30.6880733944954129No Hit
CGGAAACAACAACTGCTTGTCATTCTCTTAAACCTATTCCTACTCCTGTG30.6880733944954129No Hit
CGGAAACAACAACTGCTGGAGCATGGCAGGATAGCCTGTTCAGACCAAGT20.45871559633027525No Hit
CGGAAACAACAACTGCTGCATCCTTAGGAAGGATCAGAAACCATGCTGCC20.45871559633027525No Hit
AGCACGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT20.45871559633027525No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGACCTGCT20.45871559633027525No Hit
AGCACAGGATCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT20.45871559633027525No Hit
CGGAAACAACAACTGCTGCCCAGCACCACCCCTGCCTGGCTGGCCCTTCC20.45871559633027525No Hit
AGCACAGGACTTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT20.45871559633027525No Hit
ACACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT20.45871559633027525No Hit
CGGAAACAACAACTCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGCT20.45871559633027525No Hit
AGCACAGGACTCTACTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT20.45871559633027525No Hit
CGTTAGTGACAAGAGGGACATCTGAGAAGAGGAAGCTGTTCAGACCAAGT10.22935779816513763No Hit
AGCACAGGATCTTCTTTGGTGTAAAGAGAGTACTTTTAAAGGTCCTGCTT10.22935779816513763No Hit
CGTTAGTGACAAGAGGGACAAAGCAGAGAGAGCCAAGAAGTATGGTGGCA10.22935779816513763No Hit
ACAACAGGTCAGATGCAGGCAGGGAGAAGCAGCTCCAGGGCAGGCGTCTG10.22935779816513763No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTGCTTTTAAAGGTCCTGCT10.22935779816513763No Hit
ACAACAGGTCAATGCAGGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.22935779816513763Clontech Universal Primer Mix Long (100% over 22bp)
CGTTAGTGACAAGAGGACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.22935779816513763Clontech Universal Primer Mix Long (96% over 26bp)
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC10.22935779816513763No Hit
CGGAAACACAACTGCTGGAGCATGGCAGGATAGCCTGTTCAGACCAAGTC10.22935779816513763No Hit
CGGAAACAACAACTCTGCTCTGGAGAAAATGCTCAGGTGATGCATTCCCT10.22935779816513763No Hit
CGGAAACAACAACTGCTGCCCACACCAACACCAGCCGTGTACCAGCACTG10.22935779816513763No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGGGTACTTTTAAAGGTCCTGCT10.22935779816513763No Hit
CGGAAACAACAACTCTGCGGTCTCTGGGGACAAAACAGAACCACCCAGGC10.22935779816513763No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTATTTTTAAAGGTCCTGCT10.22935779816513763No Hit
CGTTAGTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGCTACCGTAGTA10.22935779816513763No Hit
CGGAAACAACAACTGCTGCACAGAGCTACAGTGGCTCTGAGGGTGGCGTA10.22935779816513763No Hit
AGCACCGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT10.22935779816513763No Hit
GGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT10.22935779816513763No Hit
ACAACAGGTCAGATGCAGGCGAACGGGATTCCCAGGTCCCTGCCTTTGTG10.22935779816513763No Hit
CAACAGGTCAGATGCAGGGTACATGGGAAGCAGTGGTATCAACCGCAGAG10.22935779816513763No Hit
AGCACGGACTCTTCTTTGGTGTAAAGAGAGTACTTTTAAAGGTCCTGCTT10.22935779816513763No Hit
CGGAAACAACAACTGCTGCAAAGAAGAAAAATGGATTTTGGAGTGAGAAG10.22935779816513763No Hit
AGCACAGGACTCTTCTTTGGTACAAAGAGAGTACTTTTAAAGGTCCTGCT10.22935779816513763No Hit
CGGAAACAACACAGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGCT10.22935779816513763No Hit
CGTTAGTGACAAGAGGGACATTTGAGAAGAGGAAGCTGTTCAGACCAAGT10.22935779816513763No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCGGACCAAGTCTCTGC10.22935779816513763No Hit
CGTTAGTGACAAGAGGGACATCTTTGTCTTTAGGTCACTTGTTTCTATGG10.22935779816513763No Hit
AGCGCAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT10.22935779816513763No Hit
CGGAAACAACAACTGCTGATAAGAGGAAGCTGTTCAGACCAAGTCTCTGC10.22935779816513763No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT10.22935779816513763No Hit
ACAACAGGCAGATGCAGGCGCACTGGCAAGGAGGTAGGAACCAGCCTGTT10.22935779816513763No Hit
CGTTAGTGACAAGAGGGACATCTGGGGGCATTCGTATTGCGCCGCTAGAG10.22935779816513763No Hit
CGGAAACAACAACTGCTGCCGTGTACAGTGCTGCGATTCTGGAGTACCTC10.22935779816513763No Hit
CGGAAACAACAATGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGCT10.22935779816513763No Hit
CGTTAGTGACAAGAGGGACATCGAATGTGTCCACAAAGAATCAGGTACAA10.22935779816513763No Hit
CGGAAACAACAACTGCTGCTGGGTTCCCAGAACAGAATGCACTTGTGTCC10.22935779816513763No Hit
ACACAGGTCAGATGCAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.22935779816513763Clontech Universal Primer Mix Long (100% over 23bp)
CGTTAGTGAAAGAGGGACATGGATGGAGGGAAGAGCATGGAGGGAGGGAG10.22935779816513763No Hit
CGGAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGCT10.22935779816513763No Hit
ACAACAGGTCAGATGCCATTAGTTTCAGATTAGTCTCAAGTCTACTGTAG10.22935779816513763No Hit
CGTTAGTGACAAGAGGGACAGCTGAGAAGAGGAAGCTGTTCAGACCAAGT10.22935779816513763No Hit
CGTTAGTGACAAGAGGGACATCTGGCATGACATACTCGGAGCCGTCCGGG10.22935779816513763No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGATCCTGCT10.22935779816513763No Hit
CGTTAGTGACAAGAGGGACATCTCGTCGTCCGAGCGCCTGGACATCCTGC10.22935779816513763No Hit
CGGAAACAACAACTGCTGCGTCATGGAGTACCACCAAGCCACAGGCACCC10.22935779816513763No Hit
AGCACAGGGACTCTTCTTTGGTATAAAGAGGGTACTTTTAAAGGTCCTGC10.22935779816513763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)