FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24548
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCAC886336.10477431970018No Hit
AGACCCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGC541122.04252892292651No Hit
TGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGAT350714.286296235945903No Hit
AGACCCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGC20848.489489978817012No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCAAATT10794.3954700993971No Hit
CTTCCTCTTCTCAGCAGTTGTTGTTTCCGTCCAACTGTAGAACCATGTCG2501.0184129053283364No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTGCA2360.9613817826299494No Hit
AGACCCTGGAACTGCTACATGTGCGGGCACAAGGGTACCTACGGGCTGCT910.37070229753951445No Hit
GCTATCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA850.3462603878116343No Hit
GCTATCCTGCCATGCTCCGGACACTCCTGCAGCTCCAGCTTATCATTCAC840.342186736190321No Hit
CTTCCTCTTCTCAGCTGGGACAGGTGGGTAAACCTTTGGAGGGTCCTAAG720.2933029167345608No Hit
GCTATCCTGCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA670.27293465862799415No Hit
CTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA600.2444190972788007No Hit
GTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA450.18331432295910055No Hit
GCTTCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA360.1466514583672804No Hit
GCATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATTCACA350.1425778067459671No Hit
AGACCCCTGGAACTGCTACATGTGTGGGCACAAGGGTACCTACGGGCTGC310.1262832002607137No Hit
AGACCCTGGAACTGCTACCTGTGCGGGCACAAGGGTACCTACGGGCTGCT280.11406224539677366No Hit
GCTATCCTGCCATGCTCCAGACACTCCTGCAGCTCCAGCTTATCATCCAC280.11406224539677366No Hit
TGGCTCATCTTCAACCGTCTCCTGTTTTGTAGTCCAACCCTGTGATGATT270.10998859377546033No Hit
GCTATCCTGCCATGCTCCAGACACCCCTGCAGCTCCAGCTTATCATTCAC250.10184129053283364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)