FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14867
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length143-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC597340.17622923252842No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT422928.44555054819399No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC268818.080312100625545No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC750.504472993879061No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT730.4910203807089527No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT570.3833994753480863No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT530.3564942490078698No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT380.2555996502320576No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT350.23542073047689516No Hit
CAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT320.2152418107217327No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT270.18161027779646197No Hit
AGCAAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT270.18161027779646197No Hit
AGCACAGGATCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT260.1748839712114078No Hit
ACAACAGGTCAGATGCAGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT240.16143135804129952No Hit
ACAACAGGTCAGATCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT230.15470505145624538No Hit
AGCACAGGACTTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT220.14797874487119123No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCAC220.14797874487119123No Hit
AAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT220.14797874487119123No Hit
AGACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT210.14125243828613707No Hit
ACAACAGGTAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT210.14125243828613707No Hit
AGCCAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT200.13452613170108293No Hit
ACAACGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT190.1277998251160288No Hit
ACAACAGGTCAGTGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT170.11434721194592048No Hit
ACAACAGGTCAGATGCGGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC170.11434721194592048No Hit
ACAACAGGTCAGATGCAGGTCCAGCGGTGTCCCAAGCTCCCACCCAGCGC170.11434721194592048No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT170.11434721194592048No Hit
ACACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT160.10762090536086635No Hit
ACAACAGGTCGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT160.10762090536086635No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGCGTCCCAAGCTCCCACCCAGCGC160.10762090536086635No Hit
AGCACGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT150.10089459877581221No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAGGGTCCTGCT150.10089459877581221No Hit
ACAAAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGCT150.10089459877581221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)