FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008142830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008142830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25496
Total Bases3.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA874834.31126451208033No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGC728828.584876058989643No Hit
AGCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCT278410.919359899592093No Hit
ACAACAGGTCAGATGCAGGCCCATCGGTGTCCCAAGCTCCCACCCAGCGC14995.879353624097898No Hit
CGGAAACAACAACTGCTGCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCT12174.773297772199561No Hit
CGGAAACAACAACTGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC1690.6628490743646063No Hit
CGTTAGTGACAAGGGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA1040.4079071226859115No Hit
CGTTAGTGACAAGAGGGACATTTCGCGATTTCTCGAGTCCAACCCTGTGA990.38829620332601195No Hit
ACACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT830.3255412613743332No Hit
CGTTAGTGACAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT820.3216190775023533No Hit
ACAACAGGTCAGATGCAGGCCCAGCGGTGTCCCGAGCTCCCACCCAGCGC690.27063068716661437No Hit
CGTTAGTGACAAAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT620.24317540006275495No Hit
CGTTAGTGACAAGAGGGACATCTTGCGATTTCTCGAGTCCAACCCTGTGA610.23925321619077505No Hit
CAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT550.2157201129588955No Hit
CGTTAGTGAAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT550.2157201129588955No Hit
ACAACAGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT490.192187009727016No Hit
CGTTATGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT410.16080953875117665No Hit
CGTTGGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGA410.16080953875117665No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGG380.14904298713523692No Hit
AAACAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT380.14904298713523692No Hit
CGTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT360.14119861939127706No Hit
GTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAACCCTGTGAT360.14119861939127706No Hit
GCACAGGACTCTTCTTTGGTATAAAGAGAGTACTTTTAAAGGTCCTGCTT350.13727643551929714No Hit
ACAAAGGTCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT330.1294320677753373No Hit
CGTTAGTGACAAGAGGGACTCTCGCGATTTCTCGAGTCCAACCCTGTGAT330.1294320677753373No Hit
ACAACAGGTCAGTGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT300.11766551615939756No Hit
ACAACAGGCAGATGCAGGCCCAGCGGTGTCCCAAGCTCCCACCCAGCGCT290.11374333228741763No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGCCCAACCCTGTGA280.10982114841543772No Hit
CGGAAACAACAACAGCTGAGAAGAGGAAGCTGTTCAGACCAAGTCTCTGC280.10982114841543772No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCTCGAGTCCAGCCCTGTGA280.10982114841543772No Hit
CGTTAGTGACAAGAGGGACATCTCGCGATTTCCCGAGTCCAACCCTGTGA280.10982114841543772No Hit
CGTTAGTGACAAGAGGGACATCTCGCGACTTCTCGAGTCCAACCCTGTGA260.10197678067147788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)